A Ti-plasmid-convertible .LAMBDA. Phage Vector System Harboring the Hygromycin B Phosphotransferase Gene.
نویسندگان
چکیده
منابع مشابه
Large-scale purification and acute toxicity of hygromycin B phosphotransferase.
OBJECTIVE To provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli. METHODS Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared ...
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A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B-hygromycin-B. The combined active fractions showed a single protein band (41 kDa) when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. When gel electropho...
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The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.
متن کاملThe Ti plasmid
gene can be spliced to the virus vector. Also, the added gene affects the cell-to-cell movement (invasiveness) of the virus, since the virus can no longer mature. Moreover, the virus is mainly limited to Cruciferae as host plants and therefore the prospects of infecting other major crops are narrow. Nevertheless, in-depth studies on this virus will help provide important information on means to...
متن کاملA One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles
Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format. In addition, alkali minipreps usually require additional column-based purification steps and cannot isolate other extra-chromosomal elements, such as bacteriophages. Non-ionic detergents (NIDs) have been used occasionally as co...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 1998
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.15.227